I thought it led to some interesting predictions -- for example, what happens if you ramp up NAD levels? I've never worked with C. elegans but it strikes me that this might be an easy experiment to do with them, and you'd get results pretty quickly. According to this paper, you're getting this great benefit from increased NAD levels because it's used as a cofactor by an enzyme that converts glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate, which is important because G3P (and its precursor, dihydroxyacetone phosphate) can spontaneously decompose into the toxic glycating agent methylglyoxal. So what happens if you add more NAD into a system? Or, for that matter, increase levels and/or activity of the relevant enzyme (GAPDH)?
Tuesday, November 20, 2007
Fun with NAD
I stumbled on this interesting review article a short while ago: Hipkiss, AR. Energy metabolism, altered proteins, sirtuins and ageing: converging mechanisms? http://www.springerlink.com/content/j2332796374m3178/
I thought it led to some interesting predictions -- for example, what happens if you ramp up NAD levels? I've never worked with C. elegans but it strikes me that this might be an easy experiment to do with them, and you'd get results pretty quickly. According to this paper, you're getting this great benefit from increased NAD levels because it's used as a cofactor by an enzyme that converts glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate, which is important because G3P (and its precursor, dihydroxyacetone phosphate) can spontaneously decompose into the toxic glycating agent methylglyoxal. So what happens if you add more NAD into a system? Or, for that matter, increase levels and/or activity of the relevant enzyme (GAPDH)?
I thought it led to some interesting predictions -- for example, what happens if you ramp up NAD levels? I've never worked with C. elegans but it strikes me that this might be an easy experiment to do with them, and you'd get results pretty quickly. According to this paper, you're getting this great benefit from increased NAD levels because it's used as a cofactor by an enzyme that converts glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate, which is important because G3P (and its precursor, dihydroxyacetone phosphate) can spontaneously decompose into the toxic glycating agent methylglyoxal. So what happens if you add more NAD into a system? Or, for that matter, increase levels and/or activity of the relevant enzyme (GAPDH)?
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